Tessier, Romain published the artcile“Doubly Orthogonal” Labeling of Peptides and Proteins, Quality Control of 5738-14-7, the publication is Chem (2019), 5(8), 2243-2263, database is CAplus.
Herein, we report a cysteine bioconjugation methodol. for the introduction of hypervalent iodine compounds onto biomols. Ethynylbenziodoxolones (EBXs) engage thiols in small organic mols. and cysteine-containing peptides and proteins in a fast and selective addition onto the alkynyl triple bond, resulting in stable vinylbenziodoxolone hypervalent iodine conjugates. The conjugation occurs at room temperature in an open flask under physiol. conditions. The use of an azide-bearing EBX reagent enables a “doubly orthogonal” functionalization of the bioconjugate via strain-release-driven cycloaddition and Suzuki-Miyaura cross-coupling of the vinyl hypervalent iodine bond. We successfully applied the methodol. on relevant and complex biomols., such as histone proteins. Through single-mol. experiments, we illustrated the potential of this doubly reactive bioconjugate by introducing a triplet-state quencher close to a fluorophore, which extended its lifetime by suppressing photobleaching. This work is therefore expected to find broad applications for peptide and protein functionalization.
Chem published new progress about 5738-14-7. 5738-14-7 belongs to pyrimidines, auxiliary class Pyrimidine,Amine,Alcohol,Pyrimidine, name is 2-(Dimethylamino)pyrimidine-4,6-diol, and the molecular formula is C3H6O2, Quality Control of 5738-14-7.
Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia