Tang, Qi published the artcileCharacterization of Byproducts from Chemical Syntheses of Oligonucleotides Containing 1-Methyladenine and 3-Methylcytosine, Category: pyrimidines, the publication is ACS Omega (2017), 2(11), 8205-8212, database is CAplus and MEDLINE.
Oligonucleotides serve as important tools for biol., chem., and medical research. The preparation of oligonucleotides through automated solid-phase synthesis is well established. However, identification of byproducts generated from DNA synthesis, especially from oligonucleotides containing site-specific modifications, is sometimes challenging. Typical HPLC, Mass Spectrometry (MS), and gel electrophoresis methods alone are not sufficient for characterizing unexpected byproducts, especially for those having identical or very similar mol. weight (MW) to the products. The authors used a rigorous quality control procedure to characterize byproducts generated during oligonucleotide syntheses: (1) purify oligonucleotide by different HPLC systems; (2) determine the exact MW by high resolution MS; (3) locate modification position by MS/MS or exonuclease digestion with MALDI-TOF anal.; and (4) conduct, where applicable, enzymic assays. The authors applied these steps to characterize byproducts in the syntheses of oligonucleotides containing important Me DNA adducts 1-methyladenine (m1A) and 3-methylcytosine (m3C). In m1A synthesis, the authors differentiated a regioisomeric byproduct 6-methyladenine, which possesses identical MW to m1A. As for m3C, the authors identified a deamination byproduct 3-methyluracil, which is only 1 Da greater than m3C in the ∼ 4900 Da context. The detection of these byproducts would be very challenging if the abovementioned procedure were not adopted.
ACS Omega published new progress about 608-34-4. 608-34-4 belongs to pyrimidines, auxiliary class Pyrimidine,Amide, name is 3-Methylpyrimidine-2,4(1H,3H)-dione, and the molecular formula is C14H12N2S, Category: pyrimidines.
Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia