Tag: 100-200

On December 31, 2022, Zhang, Hua; Zhao, Lin; Jiang, Jingjing; Zheng, Jie; Yang, Li; Li, Yanyan; Zhou, Jian; Liu, Tianshu; Xu, Jianmin; Lou, Wenhui; Yang, Weige; Tan, Lijie; Liu, Weiren; Yu, Yiyi; Ji, Meiling; Xu, Yaolin; Lu, Yan; Li, Xiaomu; Liu, Zhen; Tian, Rong; Hu, Cheng; Zhang, Shumang; Hu, Qinsheng; Deng, Yangdong; Ying, Hao; Zhong, Sheng; Zhang, Xingdong; Wang, Yunbing; Wang, Hua; Bai, Jingwei; Li, Xiaoying; Duan, Xiangfeng published an article.Category: pyrimidines The title of the article was Multiplexed nanomaterial-assisted laser desorption/ionization for pan-cancer diagnosis and classification. And the article contained the following:

As cancer is increasingly considered a metabolic disorder, it is postulated that serum metabolite profiling can be a viable approach for detecting the presence of cancer. By multiplexing mass spectrometry fingerprints from two independent nanostructured matrixes through machine learning for highly sensitive detection and high throughput anal., we report a laser desorption/ionization (LDI) mass spectrometry-based liquid biopsy for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) is applied in 1,183 individuals that include 233 healthy controls and 950 patients with liver, lung, pancreatic, colorectal, gastric, thyroid cancers from two independent cohorts. MNALCI demonstrates 93% sensitivity at 91% specificity for distinguishing cancers from healthy controls in the internal validation cohort, and 84% sensitivity at 84% specificity in the external validation cohort, with up to eight metabolite biomarkers identified. In addition, across those six different cancers, the overall accuracy for identifying the tumor tissue of origin is 92% in the internal validation cohort and 85% in the external validation cohort. The excellent accuracy and min. sample consumption make the high throughput assay a promising solution for non-invasive cancer diagnosis. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Category: pyrimidines

The Article related to liver lung pancreatic colorectal gastric thyroid cancer diagnosis biomarker, Biochemical Methods: Spectral and Related Methods and other aspects.Category: pyrimidines

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On March 3, 2020, Zhang, Yi; Xiao, Jing-Yu; Zhu, Yuan; Tian, Li-Jiao; Wang, Wei-Kang; Zhu, Ting-Ting; Li, Wen-Wei; Yu, Han-Qing published an article.Application of 65-71-4 The title of the article was Fluorescence Sensor Based on Biosynthetic CdSe/CdS Quantum Dots and Liposome Carrier Signal Amplification for Mercury Detection. And the article contained the following:

Mercury (Hg), as a highly harmful environmental pollutant, poses severe ecol. and health risks even at low concentrations Accurate and sensitive methods for detecting Hg2+ ions in aquatic environments are highly needed. In this work, the authors developed a highly sensitive fluorescence sensor for Hg2+ detection with an integrated use of biosynthetic CdSe/CdS quantum dots (QDs) and liposome carrier signal amplification. To construct such a sensor, three single-stranded DNA probes were rationally designed based on the thymine-Hg2+-thymine (T-Hg2+-T) coordination chem. principles and by taking advantage of the biocompatibility and facile-modification properties of the biosynthetic QDs. Hg2+ could be determined in a range from 0.25 to 100 nM with a detection limit of 0.01 nM, which met the requirements of environmental sample detection. The sensor also exhibited a high selectivity for Hg2+ detection in the presence of other high-level metal ions. A satisfactory capacity of the sensor for detecting environmental samples including tap water, river water, and landfill leachate was also demonstrated. This work opens up a new application scenario for biosynthetic QDs and holds a great potential for environmental monitoring applications. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Application of 65-71-4

The Article related to fluorescence sensor cadmium selenide sulfide quantum dot liposome mercury, Biochemical Methods: Spectral and Related Methods and other aspects.Application of 65-71-4

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 31, 2020, Dementjev, Andrej; Rutkauskas, Danielis; Polovy, Ivan; Macernis, Mindaugas; Abramavicius, Darius; Valkunas, Leonas; Dovbeshko, Galina published an article.Application of 65-71-4 The title of the article was Characterization of thymine microcrystals by CARS and SHG microscopy. And the article contained the following:

Identification of chem. homologous microcrystals in a polycrystal sample is a big challenge and requires developing specific highly sensitive tools. Second harmonic (SHG) and coherent anti-Stokes Raman scattering (CARS) spectroscopy can be used to reveal arrangement of thymine mols., one of the DNA bases, in microcrystalline sample. Strong dependence of CARS and SHG intensity on the orientation of the linear polarization of the excitation light allows to obtain high resolution images of thymine microcrystals by addnl. utilizing the scanning microscopy technique. Exptl. findings and theor. interpretation of the results are compared. Presented exptl. data together with quantum chem.-based theor. interpretation allowed us to determine the most probable organization of the thymine mols. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Application of 65-71-4

The Article related to thymine microcrystal structure cars second harmonic generation microscopy, Biochemical Methods: Spectral and Related Methods and other aspects.Application of 65-71-4

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Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Ravera, Francesca; Efeoglu, Esen; Byrne, Hugh J. published an article in 2021, the title of the article was Monitoring stem cell differentiation using Raman microspectroscopy: chondrogenic differentiation, towards cartilage formation.Electric Literature of 65-71-4 And the article contains the following content:

Mesenchymal Stem Cells (MSCs) have the ability to differentiate into chondrocytes, the only cellular components of cartilage and are therefore ideal candidates for cartilage and tissue repair technologies. Chondrocytes are surrounded by cartilage-like extracellular matrix (ECM), a complex network rich in glycosaminoglycans, proteoglycans, and collagen, which, together with a multitude of intracellular signalling mols., trigger the chondrogenesis and allow the chondroprogenitor to acquire the spherical morphol. of the chondrocytes. However, although the mechanisms of the differentiation of MSCs have been extensively explored, it has been difficult to provide a holistic picture of the process, in situ. Raman Micro Spectroscopy (RMS) has been demonstrated to be a powerful anal. tool, which provides detailed label free biochem. fingerprint information in a non-invasive way, for anal. of cells, tissues and body fluids. In this work, RMS is explored to monitor the process of Mesenchymal Stem Cell (MSC) differentiation into chondrocytes in vitro, providing a holistic mol. picture of cellular events governing the differentiation. Spectral signatures of the subcellular compartments, nucleolus, nucleus and cytoplasm were initially probed and characteristic mol. changes between differentiated and undifferentiated were identified. Moreover, high d. cell micromasses were cultured over a period of three weeks, and a systematic monitoring of cellular mol. components and the progress of the ECM formation, associated with the chondrogenic differentiation, was performed. This study shows the potential applicability of RMS as a powerful tool to monitor and better understand the differentiation pathways and process. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Electric Literature of 65-71-4

The Article related to chondrogenic stem cell differentiation raman microspectroscopy, Biochemical Methods: Spectral and Related Methods and other aspects.Electric Literature of 65-71-4

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On September 30, 2018, Pelclova, Daniela; Navratil, Tomas; Vlckova, Stepanka; Fenclova, Zdenka; Pelcl, Tomas; Kacerova, Tereza; Kacer, Petr published an article.Synthetic Route of 4433-40-3 The title of the article was Exhaled breath condensate biomarkers reflect systemic changes in patients with chronic dioxin intoxication. And the article contained the following:

Abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is highly toxic and affects the cardiovascular system, brain, and skin by AhR-dependent and other mechanisms, as well as causing metabolic impairments and cancer. The involvement of the respiratory system has not yet been studied. TCDD in the blood was measured and biomarkers of oxidative stress and inflammation were analyzed in 2016 in the exhaled breath condensate (EBC) of the last eight male survivors (mean age 72.4 years) from 80 workers intoxicated with TCDD during the production of herbicides from 1965 to 1968. The results were compared with their findings in 2010 to evaluate a trend. Malondialdehyde, 4-hydroxy-trans-nonenale, and 8-isoprostaglandin F2α (8-isoprostane), in addition to markers of the oxidation of nucleic acids and proteins 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine, 5-(hydroxymethyl)uracil, o-tyrosine, and 3-nitrotyrosine, as well as markers of inflammation leukotrienes and anti-inflammatory lipoxins, were analyzed in EBC by liquid chromatog.-electrospray ionization-tandem mass spectrometry. In addition, the patients underwent chest x-ray, spirometry and fractional exhaled nitric oxide (FeNO) examinations The control group included 7 men (66 years) with comparable lifestyle factors. The median plasma TCDD level lowered from 155 (28-553) ng/kg fat in 2010 to 112 (46-390) ng/kg fat in 2016, i.e., 50 years after exposure. The mean TCDD body deposit was 5.0 μg. Serum TCDD level in the pooled sample of the controls was 12 ng/kg fat. All markers of oxidative stress, LTB4 and LTC4, remained overexpressed in patients and anti-inflammatory lipoxins were under-expressed compared to controls (all). The mean FeNO and spirometry results were within the reference values. Borderline x-ray findings and combined lung function impairments were seen in the patients with the lower TCDD plasma levels. Differences in the expression of the biomol. markers in EBC as compared to controls were not associated with lung impairments and the respiratory parameters measured. Therefore, these EBC markers can be used to evaluate systemic oxidative stress and inflammation in tissues and the endovascular, atherosclerotic, neurotoxic, and metabolic effects of TCDD. Graphical abstract: [Figure not available: see fulltext.]. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Synthetic Route of 4433-40-3

The Article related to dioxin occupational poisoning breath condensate biomarker, Toxicology: Carcinogens, Mutagens, and Teratogens and other aspects.Synthetic Route of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On February 12, 2020, Chen, Feng; Xue, Jing; Zhang, Jin; Bai, Min; Yu, Xu; Fan, Chunhai; Zhao, Yongxi published an article.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Differentiated Visualization of Single-Cell 5-Hydroxymethylpyrimidines with Microfluidic Hydrogel Encoding. And the article contained the following:

5-Hydroxymethyluracil (5hmU) is found in the genomes of a diverse range of organisms as another kind of 5-hydroxymethylpyrimidine, with the exception of 5-hydroxymethylcytosine (5hmC). The biol. function of 5hmU has not been well explored due to lacking both specific 5hmU recognition and single-cell anal. methods. Here we report differentiated visualization of single-cell 5hmU and 5hmC with microfluidic hydrogel encoding (s.c.5hmU/5hmC-microgel). Single cells and their genomic DNA after cell lysis can be encapsulated in individual agarose microgels. The 5hmU sites are then specifically labeled with thiophosphate for the first time, followed by labeling 5hmC with azide glucose. These labeled bases are each encoded into resp. DNA barcode primers by chem. crosslinking. In situ amplification is triggered for single-mol. fluorescence visualization of single-cell 5hmU and 5hmC. On the basis of the s.c.5hmU/5hmC-microgel, we reveal cell type-specific mol. signatures of these two bases with remarkable single-cell heterogeneity. Utilizing machine learning algorithms to decode four-dimensional signatures of 5hmU/5hmC, we visualize the discrimination of nontumorigenic, carcinoma and highly invasive breast cell lines. This strategy provides a new route to analyze and decode single-cell DNA epigenetic modifications. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to fluorescent imaging cell microfluidic hydrogel, Biochemical Methods: Spectral and Related Methods and other aspects.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On March 14, 2021, Dempwolff, Adrian L.; Belogolova, Alexandra M.; Trofimov, Alexander B.; Dreuw, Andreas published an article.Recommanded Product: 65-71-4 The title of the article was Intermediate state representation approach to physical properties of molecular electron-attached states: Theory, implementation, and benchmarking. And the article contained the following:

Computational schemes for comprehensive studies of mol. electron-attached states and the calculation of electron affinities (EAs) are formulated and implemented employing the intermediate state representation (ISR) formalism and the algebraic-diagrammatic construction approximation for the electron propagator (EA-ADC). These EA-ADC(n)/ISR(m) schemes allow for a consistent treatment of not only electron affinities and pole strengths up to third-order of perturbation theory (n = 3) but also one-electron properties of electron-attached states up to second order (m = 2). The EA-ADC/ISR equations were implemented in the Q-CHEM program for Sẑ-adapted intermediate states, allowing also open-shell systems to be studied using UHF references For benchmarking of the EA-(U)ADC/ISR schemes, EAs and dipole moments of various electron-attached states of small closed- and open-shell mols. were computed and compared to full CI data. As an illustrative example, EA-ADC(3)/ISR(2) has been applied to the thymine-thymine (6-4) DNA photolesion. (c) 2021 American Institute of Physics. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Recommanded Product: 65-71-4

The Article related to intermediate state representation electron attached mol state propagator method, General Physical Chemistry: Electronic Structure and other aspects.Recommanded Product: 65-71-4

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On March 31, 2016, Pelclova, Daniela; Zdimal, Vladimir; Kacer, Petr; Fenclova, Zdenka; Vlckova, Stepanka; Syslova, Kamila; Navratil, Tomas; Schwarz, Jaroslav; Zikova, Nadezda; Barosova, Hana; Turci, Francesco; Komarc, Martin; Pelcl, Tomas; Belacek, Jaroslav; Kukutschova, Jana; Zakharov, Sergey published an article.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Oxidative stress markers are elevated in exhaled breath condensate of workers exposed to nanoparticles during iron oxide pigment production. And the article contained the following:

Markers of oxidative stress and inflammation were analyzed in the exhaled breath condensate (EBC) and urine samples of 14 workers (mean age 43 ± 7 years) exposed to iron oxide aerosol for an average of 10 ± 4 years and 14 controls (mean age 39 ± 4 years) by liquid chromatog.-electrospray ionization-mass spectrometry/mass spectrometry (LC-ESI-MS/MS) after solid-phase extraction Aerosol exposure in the workplace was measured by particle size spectrometers, a scanning mobility particle sizer (SMPS) and an aerodynamic particle sizer (APS), and by aerosol concentration monitors, P-TRAK and DustTRAK DRX. Total aerosol concentrations in workplace locations varied greatly in both time and space. The median mass concentration was 0.083 mg m-3 (IQR 0.063-0.133 mg m-3) and the median particle concentration was 66 800 particles cm-3 (IQR 16 900-86 900 particles cm-3). In addition, more than 80% of particles were smaller than 100 nm in diameter Markers of oxidative stress, malondialdehyde (MDA), 4-hydroxy-trans-hexenale (HHE), 4-hydroxy-trans-nonenale (HNE), 8-isoProstaglandin F2α (8-isoprostane) and aldehydes C6-C12, in addition to markers of nucleic acid oxidation, including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU), and of proteins, such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr), and 3-nitrotyrosine (3-NOTyr) were analyzed in EBC and urine by LC-ESI-MS/MS. Almost all markers of lipid, nucleic acid and protein oxidation were elevated in the EBC of workers comparing with control subjects. Elevated markers were MDA, HNE, HHE, C6-C10, 8-isoprostane, 8-OHdG, 8-OHG, 5-OHMeU, 3-ClTyr, 3-NOTyr, o-Tyr (all p < 0.001), and C11 (p < 0.05). Only aldehyde C12 and the pH of samples did not differ between groups. Markers in urine were not elevated. These findings suggest the adverse effects of nano iron oxide aerosol exposure and support the utility of oxidative stress biomarkers in EBC. The anal. of urine oxidative stress biomarkers does not support the presence of systemic oxidative stress in iron oxide pigment production workers. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to oxidative stress marker human ebc nanoparticle iron oxide pigment, Air Pollution and Industrial Hygiene: Industrial Hygiene and other aspects.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 31, 2022, Connell, J. R.; Benton, M. C.; Lea, R. A.; Sutherland, H. G.; Chaseling, J.; Haupt, L. M.; Wright, K. M.; Griffiths, L. R. published an article.Product Details of 65-71-4 The title of the article was Pedigree derived mutation rate across the entire mitochondrial genome of the Norfolk Island population. And the article contained the following:

Estimates of mutation rates for various regions of the human mitochondrial genome (mtGenome) vary widely, depending on whether they are inferred using a phylogenetic approach or obtained directly from pedigrees. Traditionally, only the control region, or small portions of the coding region have been targeted for anal. due to the cost and effort required to produce whole mtGenome Sanger profiles. Here, we report one of the first pedigree derived mutation rates for the entire human mtGenome. The entire mtGenome from 225 individuals originating from Norfolk Island was analyzed to estimate the pedigree derived mutation rate and compared against published mutation rates. These individuals were from 45 maternal lineages spanning 345 generational events. Mutation rates for various portions of the mtGenome were calculated Nine mutations (including two transitions and seven cases of heteroplasmy) were observed, resulting in a rate of 0.058 mutations/site/million years (95% CI 0.031-0.108). These mutation rates are approx. 16 times higher than estimates derived from phylogenetic anal. with heteroplasmy detected in 13 samples (n = 225, 5.8% individuals). Providing one of the first pedigree derived estimates for the entire mtGenome, this study provides a better understanding of human mtGenome evolution and has relevance to many research fields, including medicine, anthropol. and forensics. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Product Details of 65-71-4

The Article related to mutation pedigree mitochondrial genome heteroplasmy australia, Mammalian Biochemistry: Classical Genetics and Phylogeny and other aspects.Product Details of 65-71-4

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Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 31, 2021, Ma, Danyi; Suh, Dong Ho; Zhang, Jiaying; Chao, Yufan; Duttlinger, Alan W.; Johnson, Jay S.; Lee, Choong Hwan; Kim, Yuan H. Brad published an article.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Elucidating the involvement of apoptosis in postmortem proteolysis in porcine muscles from two production cycles using metabolomics approach. And the article contained the following:

Apoptosis has been suggested as the first step in the process of conversion of muscle into meat. While a potential role of apoptosis in postmortem proteolysis has been proposed, the underlying mechanisms by which metabolome changes in muscles would influence apoptotic and proteolytic process, leading to meat quality variation, has not been determined Here, apoptotic and proteolytic attributes and metabolomics profiling of longissimus dorsi (LD) and psoas major (PM) muscles in pigs from two different production cycles (July-Jan vs. Apr-Sep) were evaluated. The PM showed higher mitochondrial membrane permeability (MMP), concurrent with less extent of calpain-1 autolysis and troponin T degradation and higher abundance of HSP27 and αβ-crystallin compared to LD (P < 0.05). Apr-Sep muscles showed concurrence of extended apoptosis (indicated by higher MMP), calpain-1 autolysis and troponin T degradation, regardless of muscle effects (P < 0.05). Metabolomics profiling showed Apr-Sep muscles to increase in oxidative stress-related macronutrients, including 6-carbon sugars, some branched-chain AA, and free fatty acids. Antioxidant AA (His and Asp) and ascorbic acid were higher in July-Jan (P < 0.05). The results of the present study suggest that early postmortem apoptosis might be pos. associated with pro-oxidant macronutrients and neg. associated with antioxidant metabolites, consequently affecting meat quality attributes in a muscle-specific manner. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to porcine muscle metabolomic postmortem proteolysis, Food and Feed Chemistry: Meat, Eggs, Fish, and Seafood and other aspects.Safety of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia