Spratt, Thomas E.; Campbell, Colin R. published the artcile< Synthesis of Oligodeoxyribonucleotides Containing Analogs of O6-Methylguanine and Reaction with O6-Alkylguanine-DNA Alkyltransferase>, Formula: C7H8N4O, the main research area is oligodeoxyribonucleotide methylguanine duplex interaction alkyltransferase; demethylation oligodeoxyribonucleotide methylguanine duplex alkyltransferase; nucleotide oligodeoxyribo preparation interaction alkyltransferase.
O6-Alkylguanine-DNA alkyltransferase (AGT) repairs the mutagenic O6-methylguanine (O6mG) lesion by transferring a Me group from the 6-position of guanine to a cysteine residue on the protein. The simplest possible mechanism is an SN2 process in which the cysteine displaces the Me group off of the guanine in a concerted reaction. To probe the interactions between the protein and guanine leaving group, oligodeoxyribonucleotide duplexes containing analogs of O6mG were synthesized and then reacted with AGT. AGT was reacted with oligonucleotide duplexes of the sequence 5′-GGCGCTXGAGGCGTG-3′ in which X was O6mG or an analog in which X was paired with C. All detected reactions were demethylations of the oligodeoxyribonucleotides except for O6-methyl-3-deoxyguanine, which reacted in an unknown manner. The second-order rate constants obtained are reported. The large decreases in rate observed for changing the oxygen at the 6-position and the ring nitrogen at the 1-position suggest that these sites are hydrogen bond acceptors and/or proton acceptors during the reaction. The potential hydrogen bond from the protein to the 1-position of O6mG as well as the increase in rate observed for O6-methylhypoxanthine suggests that the duplex opens up in order for the reaction to occur.
Biochemistry published new progress about Demethylation. 84955-32-8 belongs to class pyrimidines, and the molecular formula is C7H8N4O, Formula: C7H8N4O.
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia