On May 31, 2017, Sheng, Xiao-Qi; Wang, Yi-Chao published an article.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Novel two-step derivation method for the synchronous analysis of inherited metabolic disorders using urine. And the article contained the following:
The aim of the present study was to conduct preliminary clin. screening and monitoring using a novel two-step derivatization process of urine in five categories of inherited metabolic disease (IMD). Urine samples (100 μl, containing 2.5 mmol/l creatinine) were taken from patients with IMDs. The collected urine was then treated using a two-step derivatization method (with oximation and silylation at room temperature), where urea and protein were removed. In the first step of the derivatization, α-ketoacids and α-aldehyde acids were prepared by oximation using novel oximation reagents. The second-step of the derivatization was that residues were silylated for anal. Urine samples were examined using gas chromatog./mass spectrometry (GC/MS) and a retention time-locking technique. The simultaneous anal. and identification of >400 metabolites in >130 types of IMD was possible from the GC/MS results, where the IMDs included phenylketonuria, ornithine trans-carbamylase deficiency, neonatal intrahepatic cholestasis caused by citrin deficiency, β-ureidopropionase deficiency and mitochondrial metabolic disorders. This method was demonstrated to have good repeatability. Considering a-ketoglutarate (α-KG) as an example, the relative standard deviations (RSDs) of the α-KG retention time and peak area were 0.8 and 3.9%, resp., the blank spiked recovery rate was between 89.6 and 99.8%, and the RSD was ≤7.5% (n=5). The method facilitates the anal. of thermally non-stable and semi-volatile metabolites in urine, and greatly expands the range of materials that can be synchronously screened by GC/MS. Furthermore, it provides a comprehensive, effective and reliable biochem. anal. platform for the pathol. research of IMDs. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
The Article related to inherited metabolic disease urine biomarker diagnosis gc ms, biomarker, inherited metabolic disorders, simultaneous, two-step derivatization, urine, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia