Sabale, Pramod M. published the artcileClickable PNA Probes for Imaging Human Telomeres and Poly(A) RNAs, SDS of cas: 169396-92-3, the publication is ACS Omega (2018), 3(11), 15343-15352, database is CAplus and MEDLINE.
The ability to bind strongly to complementary nucleic acid sequences, invade complex nucleic acid structures and resist degradation by cellular enzymes has made peptide nucleic acid (PNA) oligomers as very useful hybridization probes in mol. diagnosis. For such applications, the PNA oligomers have to be labeled with appropriate reporters as they lack intrinsic labels that can be used in biophys. assays. While solid-phase synthesis is commonly used to attach reporters onto PNA, development of milder and modular labeling methods will provide access to PNA oligomers labeled with a wider range of biophys. tags. Here, the authors describe the establishment of a post-synthetic modification strategy based on bioorthogonal chem. reactions in functionalizing PNA oligomers in solution with variety of tags. A toolbox composed of alkyne- and azide-modified monomers were site-specifically incorporated into PNA oligomers and post-synthetically click-functionalized with various tags ranging from sugar, amino acid, biotin and fluorophores by using copper(I)-catalyzed azide-alkyne cycloaddition, strain-promoted azide-alkyne cycloaddition and Staudinger ligation reactions. As a proof of utility of this method, fluorescent PNA hybridization probes were developed and used in imaging human telomeres in chromosomes and poly(A) RNAs in cells. Taken together, this simple approach of generating a wide range of functional PNA oligomers will expand the use of PNA in mol. diagnosis.
ACS Omega published new progress about 169396-92-3. 169396-92-3 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Amide,Others,PNA, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C26H26N4O7, SDS of cas: 169396-92-3.
Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia