Gackowski, Daniel; Gawronski, Maciej; Kerr, Cassandra; Radivoyevitch, Tomas; Zarakowska, Ewelina; Starczak, Marta; Abakir, Abdulkadir; Ruzov, Alexey; Maciejewski, Jaroslaw P.; Olinski, Ryszard published an article in 2020, the title of the article was Base 5-formylcytosine and 5-hydroxymethyluracil as surrogate markers of TET2 and SF3B1 mutations in myelodysplastic syndrome, respectively.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:
TET2 protein has been implicated in 5-hydroxymethyluracil generation to quantify the extent to which TET2 mutations cause deficiencies in TET2 dioxygenase activity. In the present study, the linkage between various TET2 mutations and 5-hydroxymethylcytosine, 5-formylcytosine, 5-carboxycytosine and 5-hydroxymethyluracil, and linkage between these modifications and myeloid neoplastic disease were characterized. We selected patients with a broad range of different types of TET2 alterations and detected in samples from them a broad spectrum of DNA modifications. Levels of DNA markers of TET2 activity obtained using automated two-dimensional ultra-performance liquid chromatog. tandem mass spectrometry with incorporation of stable isotope-labeled internal standards The results reavealed that the best predictor/marker of TET2 mutations in patients with myeloid malignancies was 5-formylcytosine. Receiver operating characteristic (ROC) curves for predicting TET2 mutated vs. wild-type subjects show that a 5-formylcytosine threshold of 0.204 per 106dN yields a sensitivity of 100% and a specificity of 83%. Disruption of SF3B1 in the K562 cell line resulted in G2/M cell cycle arrest. In conclusion, our results suggested that the anal. of 5-formylcytosine and 5-hydroxymethyluracil are warranted as surrogate markers of TET2 and SF3B1 mutations in myelodysplastic syndrome . The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
The Article related to formylcytosine hydroxymethyluracil tet2 sf3b1 mutation myelodysplastic syndrome, Mammalian Pathological Biochemistry: Oncology and other aspects.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia