On February 12, 2020, Chen, Feng; Xue, Jing; Zhang, Jin; Bai, Min; Yu, Xu; Fan, Chunhai; Zhao, Yongxi published an article.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Differentiated Visualization of Single-Cell 5-Hydroxymethylpyrimidines with Microfluidic Hydrogel Encoding. And the article contained the following:
5-Hydroxymethyluracil (5hmU) is found in the genomes of a diverse range of organisms as another kind of 5-hydroxymethylpyrimidine, with the exception of 5-hydroxymethylcytosine (5hmC). The biol. function of 5hmU has not been well explored due to lacking both specific 5hmU recognition and single-cell anal. methods. Here we report differentiated visualization of single-cell 5hmU and 5hmC with microfluidic hydrogel encoding (s.c.5hmU/5hmC-microgel). Single cells and their genomic DNA after cell lysis can be encapsulated in individual agarose microgels. The 5hmU sites are then specifically labeled with thiophosphate for the first time, followed by labeling 5hmC with azide glucose. These labeled bases are each encoded into resp. DNA barcode primers by chem. crosslinking. In situ amplification is triggered for single-mol. fluorescence visualization of single-cell 5hmU and 5hmC. On the basis of the s.c.5hmU/5hmC-microgel, we reveal cell type-specific mol. signatures of these two bases with remarkable single-cell heterogeneity. Utilizing machine learning algorithms to decode four-dimensional signatures of 5hmU/5hmC, we visualize the discrimination of nontumorigenic, carcinoma and highly invasive breast cell lines. This strategy provides a new route to analyze and decode single-cell DNA epigenetic modifications. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
The Article related to fluorescent imaging cell microfluidic hydrogel, Biochemical Methods: Spectral and Related Methods and other aspects.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia