Category: pyrimidines

Zhou, Shengwang published the artcilePhotoactivatable Reaction for Covalent Nanoscale Patterning of Multiple Proteins, Category: pyrimidines, the publication is ACS Applied Materials & Interfaces (2018), 10(47), 40452-40459, database is CAplus and MEDLINE.

This article describes a photochem. approach for independently patterning multiple proteins to an inert substrate, particularly for studies of cell adhesion. A photoactivatable chloropyrimidine ligand was employed for covalent immobilization of SnapTag fusion proteins on self-assembled monolayers of alkanethiolates on gold. A two-step procedure was used: first, patterned UV illumination of the surface activated protein capture ligands, and second, incubation with a SnapTag fusion protein bound to the surface in illuminated regions. Two different fluorescent proteins were patterned in registry with features of 400 nm in size over a 1 mm² area. An example is given wherein an anti-carcinoembryonic antigen (anti-CEA) scFv antibody was patterned to direct the selective attachment of a human cancer cell line that express the CEA antigen. This method enables the preparation of surfaces with control over the d. and activity of independently patterned proteins.

ACS Applied Materials & Interfaces published new progress about 56-05-3. 56-05-3 belongs to pyrimidines, auxiliary class Pyrimidine,Chloride,Amine,API, name is 2-Amino-4,6-dichloropyrimidine, and the molecular formula is C25H29N9O3, Category: pyrimidines.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Lygina, Antonina S. published the artcileTransmembrane Domain Peptide/Peptide Nucleic Acid Hybrid as a Model of a SNARE Protein in Vesicle Fusion, Recommanded Product: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido)acetic acid, the publication is Angewandte Chemie, International Edition (2011), 50(37), 8597-8601, S8597/1-S8597/15, database is CAplus and MEDLINE.

A novel simplified model was introduced for membrane fusion mediated by SNARE proteins. The SNARE mimetic system consisted of hybrids between the transmembrane domain/linker segments from natural membrane-bound SNARE proteins and peptide nucleic acid recognition motifs. Owing to the complementarity of the PNA bas pairs, it was possible to investigate the fusion complexes with both helixes linked on the same and opposite sides of the recognition motif. The new PNA/peptide hybrids facilitated fusion of vesicles dependent on the PNA base pair sequence and on temperature

Angewandte Chemie, International Edition published new progress about 169396-92-3. 169396-92-3 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Amide,Others,PNA, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C26H26N4O7, Recommanded Product: 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido)acetic acid.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Lygina, Antonina S. published the artcileTransmembrane Domain Peptide/Peptide Nucleic Acid Hybrid as a Model of a SNARE Protein in Vesicle Fusion, Formula: C39H35N5O8, the publication is Angewandte Chemie, International Edition (2011), 50(37), 8597-8601, S8597/1-S8597/15, database is CAplus and MEDLINE.

A novel simplified model was introduced for membrane fusion mediated by SNARE proteins. The SNARE mimetic system consisted of hybrids between the transmembrane domain/linker segments from natural membrane-bound SNARE proteins and peptide nucleic acid recognition motifs. Owing to the complementarity of the PNA bas pairs, it was possible to investigate the fusion complexes with both helixes linked on the same and opposite sides of the recognition motif. The new PNA/peptide hybrids facilitated fusion of vesicles dependent on the PNA base pair sequence and on temperature

Angewandte Chemie, International Edition published new progress about 186046-81-1. 186046-81-1 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(4-(((benzhydryloxy)carbonyl)amino)-2-oxopyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C39H35N5O8, Formula: C39H35N5O8.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Sulkowska, A. published the artcileProton NMR studies on the interaction of alkyl derivatives of pyrimidine bases, their nucleosides and nucleotides with bovine serum albumin, Product Details of C5H6N2O2, the publication is Journal of Molecular Structure (1995), 73-6, database is CAplus.

Besides well known structural units rare purine and pyrimidine bases are found in some nucleic acids. Most of them are Me derivatives of the major bases, but some contain acetyl, isopentenyl, or hydroxymethyl groups. Rare bases are especially prominent in tRNAs which characteristically contain up to 10 percent of these unusual components. The aim of this study was to provide by ¹H NMR spectroscopy a detailed exptl. base for understanding the nature of hydrophobic complexes formed by pyrimidine bases, their nucleosides and nucleotides when they interact with protein. As the model protein bovine serum albumin (BSA) has been selected. Addition of serum albumin caused a broadening of the NMR signals.

Journal of Molecular Structure published new progress about 608-34-4. 608-34-4 belongs to pyrimidines, auxiliary class Pyrimidine,Amide, name is 3-Methylpyrimidine-2,4(1H,3H)-dione, and the molecular formula is C6H9N3O2, Product Details of C5H6N2O2.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Li, Jian-Yuan published the artcilePalladium-Catalyzed Hydroxycarbonylation of (Hetero)aryl Halides for DNA-Encoded Chemical Library Synthesis, Category: pyrimidines, the publication is Bioconjugate Chemistry (2019), 30(8), 2209-2215, database is CAplus and MEDLINE.

A strategy for DNA-compatible, palladium-catalyzed hydroxycarbonylation of (hetero)aryl halides on DNA-chem. conjugates has been developed. This method generally provided the corresponding carboxylic acids in moderate to very good conversions for (hetero)aryl iodides and bromides, and in poor to moderate conversions for (hetero)aryl chlorides. These conditions were further validated by application within a DNA-encoded chem. library synthesis and subsequent discovery of enriched features from the library in selection experiments against two protein targets.

Bioconjugate Chemistry published new progress about 56-05-3. 56-05-3 belongs to pyrimidines, auxiliary class Pyrimidine,Chloride,Amine,API, name is 2-Amino-4,6-dichloropyrimidine, and the molecular formula is C4H3Cl2N3, Category: pyrimidines.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Sharif, Ehesan U. published the artcileDevelopment of a Scalable and Practical Synthesis of AB928, a Dual A_2a/A_2b Receptor Antagonist, Synthetic Route of 56-05-3, the publication is Organic Process Research & Development (2020), 24(7), 1254-1261, database is CAplus.

First- and second-generation routes to the dual A_2a/A_2b receptor antagonist AB928 I are disclosed. The second-generation route (performed on multigram scale) prepared the aminochloropyridinylbenzonitrile II by a route avoiding palladium-catalyzed borylation and Suzuki coupling reactions required in the initial route which generated significant amounts of byproducts.

Organic Process Research & Development published new progress about 56-05-3. 56-05-3 belongs to pyrimidines, auxiliary class Pyrimidine,Chloride,Amine,API, name is 2-Amino-4,6-dichloropyrimidine, and the molecular formula is C9H21NO3, Synthetic Route of 56-05-3.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Li, Nan published the artcileCopper-Free Sonogashira Cross-Coupling for Functionalization of Alkyne-Encoded Proteins in Aqueous Medium and in Bacterial Cells, Synthetic Route of 5738-14-7, the publication is Journal of the American Chemical Society (2011), 133(39), 15316-15319, database is CAplus and MEDLINE.

Bioorthogonal reactions suitable for functionalization of genetically or metabolically encoded alkynes, for example, copper-catalyzed azide-alkyne cycloaddition reaction (“click chem.”), have provided chem. tools to study biomol. dynamics and function in living systems. Despite its prominence in organic synthesis, copper-free Sonogashira cross-coupling reaction suitable for biol. applications has not been reported. In this work, the authors report the discovery of a robust aminopyrimidine-palladium(II) complex for copper-free Sonogashira cross-coupling that enables selective functionalization of a homopropargylglycine (HPG)-encoded ubiquitin protein in aqueous medium. A wide range of aromatic groups including fluorophores and fluorinated aromatic compounds can be readily introduced into the HPG-containing ubiquitin under mild conditions with good to excellent yields. The suitability of this reaction for functionalization of HPG-encoded ubiquitin in Escherichia coli was also demonstrated. The high efficiency of this new catalytic system should greatly enhance the utility of Sonogashira cross-coupling in bioorthogonal chem.

Journal of the American Chemical Society published new progress about 5738-14-7. 5738-14-7 belongs to pyrimidines, auxiliary class Pyrimidine,Amine,Alcohol,Pyrimidine, name is 2-(Dimethylamino)pyrimidine-4,6-diol, and the molecular formula is C6H9N3O2, Synthetic Route of 5738-14-7.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Lores Lareo, Pablo published the artcileNucleic acid and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry, Product Details of C26H26N4O7, the publication is Journal of Mass Spectrometry (2019), 54(8), 676-683, database is CAplus and MEDLINE.

Detection of nucleic acids and single nucleotide polymorphisms (SNPs) is of pivotal importance in biol. and medicine. Given that the biol. effect of SNPs often is enhanced in combination with other SNPs, multiplexed SNP detection is desirable. We show proof of concept of the multiplexed detection of SNPs based on the template-directed native chem. ligation (NCL) of PNA-probes carrying a metal tag allowing detection using ICP-MS. For the detection of ssDNA oligonucleotides (30 bases), two probes, one carrying the metal tag and a second one carrying biotin for purification, are covalently ligated. The methodol. limit of detection is of 29 pM with RSD of 6.7% at 50 pM (n = 5). Detection of SNPs is performed with the combination of two sets of reporter probes. The first probe set targets the SNP, and its yield is compared with a second set of probes targeting a neighboring sequence. The assay was used to simultaneously differentiate between alleles of three SNPs at 5-nM concentration

Journal of Mass Spectrometry published new progress about 169396-92-3. 169396-92-3 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Amide,Others,PNA, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C26H26N4O7, Product Details of C26H26N4O7.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Lores Lareo, Pablo published the artcileNucleic acid and SNP detection via template-directed native chemical ligation and inductively coupled plasma mass spectrometry, COA of Formula: C39H35N5O8, the publication is Journal of Mass Spectrometry (2019), 54(8), 676-683, database is CAplus and MEDLINE.

Detection of nucleic acids and single nucleotide polymorphisms (SNPs) is of pivotal importance in biol. and medicine. Given that the biol. effect of SNPs often is enhanced in combination with other SNPs, multiplexed SNP detection is desirable. We show proof of concept of the multiplexed detection of SNPs based on the template-directed native chem. ligation (NCL) of PNA-probes carrying a metal tag allowing detection using ICP-MS. For the detection of ssDNA oligonucleotides (30 bases), two probes, one carrying the metal tag and a second one carrying biotin for purification, are covalently ligated. The methodol. limit of detection is of 29 pM with RSD of 6.7% at 50 pM (n = 5). Detection of SNPs is performed with the combination of two sets of reporter probes. The first probe set targets the SNP, and its yield is compared with a second set of probes targeting a neighboring sequence. The assay was used to simultaneously differentiate between alleles of three SNPs at 5-nM concentration

Journal of Mass Spectrometry published new progress about 186046-81-1. 186046-81-1 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(4-(((benzhydryloxy)carbonyl)amino)-2-oxopyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C39H35N5O8, COA of Formula: C39H35N5O8.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia

Heemstra, Jennifer M. published the artcileTemplated Synthesis of Peptide Nucleic Acids via Sequence-Selective Base-Filling Reactions, Formula: C39H35N5O8, the publication is Journal of the American Chemical Society (2009), 131(32), 11347-11349, database is CAplus and MEDLINE.

The templated synthesis of nucleic acids has previously been achieved through the backbone ligation of preformed nucleotide monomers or oligomers. In contrast, the authors, here, demonstrate templated nucleic acid synthesis using a base-filling approach in which individual bases are added to abasic sites of a peptide nucleic acid (PNA). Because nucleobase substrates in this approach are not self-reactive, a base-filling approach may reduce the formation of non-templated reaction products. Using either reductive amination or amine acylation chemistries, the authors observed efficient and selective addition of each of the four nucleobases to an abasic site in the middle of the PNA strand. The authors also describe the addition of single nucleobases to the end of a PNA strand through base filling, as well as the tandem addition of two bases to the middle of the PNA strand. These findings represent an exptl. foundation for nonenzymic information transfer through base filling.

Journal of the American Chemical Society published new progress about 186046-81-1. 186046-81-1 belongs to pyrimidines, auxiliary class Pyrimidine,Carboxylic acid,Amine,Benzene,Amide,Others,PNA,, name is 2-(N-(2-((((9H-Fluoren-9-yl)methoxy)carbonyl)amino)ethyl)-2-(4-(((benzhydryloxy)carbonyl)amino)-2-oxopyrimidin-1(2H)-yl)acetamido)acetic acid, and the molecular formula is C39H35N5O8, Formula: C39H35N5O8.

Referemce:
https://pubchem.ncbi.nlm.nih.gov/compound/Pyrimidine,
Pyrimidine – Wikipedia