Category: 4433-40-3

On December 18, 2020, Nguyen, Kim; Kubota, Miles; Arco, Jon del; Feng, Chao; Singha, Monika; Beasley, Samantha; Sakr, Jasmine; Gandhi, Sunil P.; Blurton-Jones, Matthew; Fernandez Lucas, Jesus; Spitale, Robert C. published an article.COA of Formula: C5H6N2O3 The title of the article was A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. And the article contained the following:

Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochem. research. Bioorthogonal nucleosides can be used to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, the authors design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).COA of Formula: C5H6N2O3

The Article related to rna expression vinyl uracil metabolic labeling, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.COA of Formula: C5H6N2O3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On May 31, 2021, Tang, Lei published an article.Recommanded Product: 4433-40-3 The title of the article was Walking through chromatin modifications. And the article contained the following:

The Cell-TALKING technique probes DNA modifications around a histone modification in fixed cells. Chromatin is a highly organized structure composed of repeating DNA-histone complexes decorated with DNA modifications and histone post-translational modifications (PTMs). To study the composition of chromatin modifications, Zhao’s lab developed Cell-TALKING, a method that leverages DNA walking to index one-to-many combinations of a histone modification with various DNA modifications within a 10-nm nanoenvironment. In Cell-TALKING, the PTM target site is labeled with a DNA walking probe (WP), and DNA modifications including 5-hydroxymethyluracil (5hmU), 5-hydroxymethylcytosine (5hmC) and 5-formyluracil (5fU) are labeled with unique barcoding probes (BPs) that are inactivated with blocked 3′ ends. The researchers applied Cell-TALKING to study the changes in chromatin modifications during the cell cycle and identified combinations of DNA modifications and histone modifications near each other. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Recommanded Product: 4433-40-3

The Article related to cell talking chromatin modification, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Recommanded Product: 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On February 15, 2017, Nguyen, Kim; Fazio, Michael; Kubota, Miles; Nainar, Sarah; Feng, Chao; Li, Xiang; Atwood, Scott X.; Bredy, Timothy W.; Spitale, Robert C. published an article.Formula: C5H6N2O3 The title of the article was Cell-Selective Bioorthogonal Metabolic Labeling of RNA. And the article contained the following:

Stringent chem. methods to profile RNA expression within discrete cellular populations remains a key challenge in biol. To address this issue, the authors developed a chem.-genetic strategy for metabolic labeling of RNA. Cell-specific labeling of RNA can be profiled and imaged using bioorthogonal chem. The authors anticipate that this platform will provide the community with a much-needed chem. toolset for cell-type specific profiling of cell-specific transcriptomes derived from complex biol. systems. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Formula: C5H6N2O3

The Article related to cell bioorthogonal labeling rna, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Formula: C5H6N2O3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Hao, Yiming; Yuan, Xue; Qian, Peng; Bai, Guanfeng; Wang, Yiqin published an article in 2017, the title of the article was The serum analysis of dampness syndrome in patients with coronary heart disease and chronic renal failure based on the theory of “same syndromes in different diseases”.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:

Aim. To analyze the serum metabolites in patients with coronary heart disease (CHD) showing dampness syndrome and patients with chronic renal failure (CRF) showing dampness syndrome and to seek the substance that serves as the underlying basis of dampness syndrome in “same syndromes in different diseases.” Methods. Metabolic spectrum by GC-MS was performed using serum samples from 29 patients with CHD showing dampness syndrome and 32 patients with CRF showing dampness syndrome. The principal component anal. and statistical anal. of partial least squares were performed to detect the metabolites with different levels of expression in patientswith CHDand CRF. Furthermore, by comparing the VIP value and data mining inMETLIN and HMDB, we identified the common metabolites in both patient groups. Results. (1) Ten differential metabolites were found in patients with CHD showing dampness syndrome when compared to healthy subjects. Meanwhile, nine differential metabolites were found in patients with CRF showing dampness syndrome when compared to healthy subjects. (2) There were 9 differential metabolites identified when the serum metabolites of the CHD patients with dampness syndrome were compared to those of CRF patients with dampness syndrome.There were 4 common metabolites found in the serums of both patient groups. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to human chd renal failure serum analysis dampness syndrome, Mammalian Pathological Biochemistry: Cardiovascular Diseases and other aspects.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Fugger, Kasper; Bajrami, Ilirjana; Silva Dos Santos, Mariana; Young, Sarah Jane; Kunzelmann, Simone; Kelly, Geoff; Hewitt, Graeme; Patel, Harshil; Goldstone, Robert; Carell, Thomas; Boulton, Simon J.; MacRae, James; Taylor, Ian A.; West, Stephen C. published an article in 2021, the title of the article was Targeting the nucleotide salvage factor DNPH1 sensitizes BRCA-deficient cells to PARP inhibitors.Product Details of 4433-40-3 And the article contains the following content:

Mutations in the BRCA1 or BRCA2 tumor suppressor genes predispose individuals to breast and ovarian cancer. In the clinic, these cancers are treated with inhibitors that target poly(ADP-ribose) polymerase (PARP). We show that inhibition of DNPH1, a protein that eliminates cytotoxic nucleotide 5-hydroxymethyl-deoxyuridine (hmdU) monophosphate, potentiates the sensitivity of BRCA-deficient cells to PARP inhibitors (PARPi). Synthetic lethality was mediated by the action of SMUG1 glycosylase on genomic hmdU, leading to PARP trapping, replication fork collapse, DNA break formation, and apoptosis. BRCA1-deficient cells that acquired resistance to PARPi were resensitized by treatment with hmdU and DNPH1 inhibition. Because genomic hmdU is a key determinant of PARPi sensitivity, targeting DNPH1 provides a promising strategy for the hypersensitization of BRCA-deficient cancers to PARPi therapy. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Product Details of 4433-40-3

The Article related to dnph1 brca parp inhibitor sensitizer nucleotide sanitizer smug1, Pharmacology: Effects Of Neoplasm Inhibitors and Cytotoxic Agents and other aspects.Product Details of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On January 1, 2018, Aybastier, Onder; Dawbaa, Sam; Demir, Cevdet published an article.Related Products of 4433-40-3 The title of the article was Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry. And the article contained the following:

Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different anal. methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts Total phenol contents were 107.39 ± 8.94 mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32 ± 10.66 mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Related Products of 4433-40-3

The Article related to dna oxidative damage grape seed extract antioxidant, antioxidant, catechin, cyanidin, dna oxidation, gc–ms/ms, grape seed, Food and Feed Chemistry: Fruits, Vegetables, Legumes, and Nuts and other aspects.Related Products of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On October 26, 2016, Shen, Weifeng; Han, Wei; Li, Yunong; Meng, Zhiqi; Cai, Leiming; Li, Liang published an article.Computed Properties of 4433-40-3 The title of the article was Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics. And the article contained the following:

Silkworm (Bombyx mori) is a very useful target insect for evaluation of endocrine disruptor chems. (EDCs) due to mature breeding techniques, complete endocrine system and broad basic knowledge on developmental biol. Comparative metabolomics of silkworms with and without EDC exposure offers another dimension of studying EDCs. The authors report a workflow on metabolomic profiling of silkworm hemolymph based on high-performance chem. isotope labeling (CIL) liquid chromatog. mass spectrometry (LC-MS) and demonstrate its application in studying the metabolic changes associated with the pesticide dichlorodiphenyltrichloroethane (DDT) exposure in silkworm. Hemolymph samples were taken from mature silkworms after growing on diet that contained DDT at four different concentrations (1, 0.1, 0.01, 0.001 ppm) as well as on diet without DDT as controls. They were subjected to differential 12C-/13C-dansyl labeling of the amine/phenol submetabolome, LC-UV quantification of the total amount of labeled metabolites for sample normalization, and LC-MS detection and relative quantification of individual metabolites in comparative samples. The total concentration of labeled metabolites did not show any significant change between four DDT-treatment groups and one control group. Multivariate statistical anal. of the metabolome data set showed that there was a distinct metabolomic separation between the five groups. Out of the 2044 detected peak pairs, 338 and 1471 metabolites have been putatively identified against the HMDB database and the EML library, resp. 65 metabolites were identified by the dansyl library searching based on the accurate mass and retention time. Among the 65 identified metabolites, 33 pos. metabolites had changes of >1.20-fold or <0.83-fold in one or more groups with p-value of smaller than 0.05. Several useful biomarkers including serine, methionine, tryptophan, asym. dimethylarginine, N-Methyl-D-aspartic and tyrosine were identified. The changes of these biomarkers were likely due to the disruption of the endocrine system of silkworm by DDT. This work illustrates that the method of CIL LC-MS is useful to generate quant. submetabolome profiles from a small volume of silkworm hemolymph with much higher coverage than conventional LC-MS methods, thereby facilitating the discovery of potential metabolite biomarkers related to EDC or other chem. exposure. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3

The Article related to isotope labeling hplc mass spectrometry silkworm hemolymph metabolomics, isotope labeling, liquid chromatography, mass spectrometry, metabolomics, pesticide, silkworm, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Computed Properties of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On May 31, 2017, Sheng, Xiao-Qi; Wang, Yi-Chao published an article.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Novel two-step derivation method for the synchronous analysis of inherited metabolic disorders using urine. And the article contained the following:

The aim of the present study was to conduct preliminary clin. screening and monitoring using a novel two-step derivatization process of urine in five categories of inherited metabolic disease (IMD). Urine samples (100 μl, containing 2.5 mmol/l creatinine) were taken from patients with IMDs. The collected urine was then treated using a two-step derivatization method (with oximation and silylation at room temperature), where urea and protein were removed. In the first step of the derivatization, α-ketoacids and α-aldehyde acids were prepared by oximation using novel oximation reagents. The second-step of the derivatization was that residues were silylated for anal. Urine samples were examined using gas chromatog./mass spectrometry (GC/MS) and a retention time-locking technique. The simultaneous anal. and identification of >400 metabolites in >130 types of IMD was possible from the GC/MS results, where the IMDs included phenylketonuria, ornithine trans-carbamylase deficiency, neonatal intrahepatic cholestasis caused by citrin deficiency, β-ureidopropionase deficiency and mitochondrial metabolic disorders. This method was demonstrated to have good repeatability. Considering a-ketoglutarate (α-KG) as an example, the relative standard deviations (RSDs) of the α-KG retention time and peak area were 0.8 and 3.9%, resp., the blank spiked recovery rate was between 89.6 and 99.8%, and the RSD was ≤7.5% (n=5). The method facilitates the anal. of thermally non-stable and semi-volatile metabolites in urine, and greatly expands the range of materials that can be synchronously screened by GC/MS. Furthermore, it provides a comprehensive, effective and reliable biochem. anal. platform for the pathol. research of IMDs. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to inherited metabolic disease urine biomarker diagnosis gc ms, biomarker, inherited metabolic disorders, simultaneous, two-step derivatization, urine, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Rozalski, Rafal; Gackowski, Daniel; Siomek-Gorecka, Agnieszka; Banaszkiewicz, Zbigniew; Olinski, Ryszard published an article in 2016, the title of the article was Urinary Measurement of Epigenetic DNA Modifications: A Non-Invasive Assessment of the Whole-Body Epigenetic Status in Healthy Subjects and Colorectal Cancer Patients.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:

Active mechanism of DNA demethylation can be responsible for the activation of previously silenced genes. Products of 5-methylcytosine oxidation are released into the bloodstream and eventually excreted with urine. Therefore, whole-body epigenetic status can be assessed non-invasively on the basis of the urinary excretion of a broad spectrum of epigenetic modifications: 5-hydroxymethylcytosine (5-hmCyt), 5-formylcytosine (5-fCyt), 5-carboxycytosine (5-caCyt), and 5-hydroxymethyluracil (5-hmUra). We have developed a specific and sensitive, isotope-dilution, automated, online, two-dimensional ultra-performance liquid chromatog. system with tandem mass spectrometry (2D UPLC-MS/MS) to measure 5-hmCyt, 5-fCyt, 5-caCyt, and their deoxynucleosides in the same urine sample. Human urine contains all of the modifications except from 5-formyl-2′-deoxycytidine (5-fdC) and 5-carboxy-2′-deoxycytidine (5-cadC). A highly significant difference in the urinary excretion of 5-(hydroxymethyl)-2′-deoxycytidine (5-hmdC) was found between healthy subjects and colorectal cancer patients (3.5 vs. 7.8 nmol mmol-1 creatinine, resp.), as well as strong correlations between the majority of analyzed compounds The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to liquid chromatog mass spectrometry epigenetic dna colorectal cancer urine, 2d uplc–ms/ms, dna damage, dna methylation, epigenetics, urine, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Mattelaer, H.-P.; Mattelaer, C.-A.; Papastavrou, N.; Dehaen, W.; Herdewijn, P. published an article in 2017, the title of the article was Oligonucleotide promoted peptide bond formation using a tRNA mimicking approach.Computed Properties of 4433-40-3 And the article contains the following content:

TransferRNA’s role in protein translation is the prime example of an Informational Leaving Group (ILG). A simplified model produced oligophenylalanine with a modified uracil as an ILG in the presence of specific oligonucleotides. Our preliminary studies contribute to the importance of hybrid species in bridging the gap between peptides and nucleic acids. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3

The Article related to peptide bond formation informational leaving group oligonucleotide photolysis, oligophenylalanine uracil synthesis hybrid species aminolysis kinetics ph peptidomimetic, Amino Acids, Peptides, and Proteins: Poly(Amino Acids) and Peptides and other aspects.Computed Properties of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia