Category: 4433-40-3

On September 30, 2020, Yang, Sha; Wang, Xiaoning; Duan, Cancan; Zhang, Jianyong published an article.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was A novel approach combining metabolomics and molecular pharmacology to study the effect of Gei Herba on mouse hematopoietic function. And the article contained the following:

Gei Herba, Chinese named Lanbuzheng (LBZ), is a traditional Chinese medicine promotes hematopoiesis, yet the underlying mechanism for this effect remains largely unknown. In the present study, a novel approach combining LC-MS metabolomics and mol. pharmacol. was developed to investigate the hematopoietic effect and mechanism of LBZ on hematopoietic dysfunction (HD) caused by cyclophosphamide (CTX) in treated mice. The results show that LBZ can reduce damage in the spleen, a result consistent with the peripheral hemogram. Fourteen potential biomarkers were identified in the spleen by metabolic profiles anal., including 5-hydroxymethyluracil, ascorbalamic acid, AMP, menadiol disulfate, L-homocysteine sulfonic acid and L-carnitine. Change in biomarker levels suggest that LBZ mainly affects β-oxidation of very-long-chain fatty acids, oxidation of branched chain fatty acids and carnitine synthesis, and those metabolites produced along with related metabolic pathways are closely associated with anti-apoptosis. A mol. pharmacol. approach was simultaneously developed to examine accompanying cellular signaling mechanisms. LBZ activates PI3K/Akt signaling pathways and granulocyte-colony-stimulating-factor (G-CSF)-mediated Janus kinase 2 (JAK2)/transcription 3 (STAT3), resulting in inhibiting the release of cytochrome c. Further, LBZ inhibits caspase-mediated mitochondrial-dependent apoptosis mediated by caspase-9 and caspase-3. LBZ can thus reduce CTX-induced HD via G-CSF-mediated JAK2/STAT3 signaling and PI3K/Akt mitochondrial-dependent apoptotic pathways. The present study combines metabolomic and mol. pharmacol. methods to elucidate mechanisms for the protective effect of LBZ on mouse HD following CTX-induced damage. This approach may be useful for exploring mechanisms of action of other drugs. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to metabolomics mol pharmacol hematopoietic function mouse, bio-markers, gei herba, hematopoietic function, metabolomics, molecular pharmacology, Placeholder for records without volume info and other aspects.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Krell, Katja; Achim-Wagenknecht, Hans published an article in 2020, the title of the article was Fluorogenic and bioorthogonal modification of RNA using photoclick chemistry.Computed Properties of 4433-40-3 And the article contains the following content:

A bromoaryltetrazole-modified uridine was synthesized as new RNA building block for bioorthogonal, light-activated and postsynthetic modification with com. available fluorescent dyes. It allows “photoclick”-type modifications by irradiation with light (300 nm LED) at internal and terminal positions of presynthesized RNA with maleimide-conjugated fluorophores in good yields. The reaction was evidenced for three different dyes. During irradiation, the emission increases due to the formation of an intrinsically fluorescent pyrazoline moiety as photoclick product. The fluorogenecity of the photoclick reaction was significantly enhanced by energy transfer between the pyrazoline as the reaction product (poor emitter) and the photoclicked dye as the strong emitter. The RNA-dye conjugates show remarkable fluorescent properties, in particular an up to 9.4 fold increase of fluorescence, which are important for chem. biol. and fluorescent imaging of RNA in cells. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3

The Article related to phosphoramidite tetrazole rna fluorescent bioorthogonal photoclick chem, oligonucleotide, photochemistry, tetrazole, Placeholder for records without volume info and other aspects.Computed Properties of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On August 20, 2021, Peach, Jesse T.; Wilson, Stephanie M.; Gunderson, Logan D.; Frothingham, Lizzi; Tran, Tan; Walk, Seth T.; Yeoman, Carl J.; Bothner, Brian; Miles, Mary P. published an article.HPLC of Formula: 4433-40-3 The title of the article was Temporal metabolic response yields a dynamic biosignature of inflammation. And the article contained the following:

Chronic low-grade inflammation is a subclin. condition directly and indirectly linked to the development of a wide range of diseases responsible for the vast majority of morbidity. To examine mechanisms coupled to chronic disease, a group of overweight and obese human subjects without known inflammatory diseases participated in a high-fat meal challenge as an acute inflammation stimulus. Anal. of serum metabolites grouped by baseline cytokine levels revealed that single samples had little power in differentiating groups. However, an anal. that incorporated temporal response separated inflammatory response phenotypes and allowed us to create a metabolic signature of inflammation which revealed metabolic components that are crucial to a cytokine-mediated inflammation response. The use of temporal response, rather than a single time point, improved metabolomic prediction of high postprandial inflammation responses and led to the development of a dynamic biosignature as a potential tool for stratifying risk to a wide range of diseases. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).HPLC of Formula: 4433-40-3

The Article related to metabolic response biosignature inflammation, metabolomics, pathophysiology, systems biology, Placeholder for records without volume info and other aspects.HPLC of Formula: 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On April 13, 2021, Pandey, Renu; Collins, Meghan; Lu, Xiyuan; Sweeney, Shannon R.; Chiou, Jennifer; Lodi, Alessia; Tiziani, Stefano published an article.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Novel Strategy for Untargeted Chiral Metabolomics using Liquid Chromatography-High Resolution Tandem Mass Spectrometry. And the article contained the following:

Stereospecific recognition of metabolites plays a significant role in the detection of potential disease biomarkers thereby providing new insights in diagnosis and prognosis. D-Hdroxy/amino acids are recognized as potential biomarkers in several metabolic disorders. Despite continuous advances in metabolomics technologies, the simultaneous measurement of different classes of enantiomeric metabolites in a single anal. run remains challenging. Here, we develop a novel strategy for untargeted chiral metabolomics of hydroxy/amine groups (-OH/-NH2) containing metabolites, including all hydroxy acids (HAs) and amino acids (AAs), by chiral derivatization coupled with liquid chromatog.-high resolution tandem mass spectrometry (LC-HR-MS/MS). Diacetyl-tartaric anhydride (DATAN) was used for the simultaneous derivatization of-OH/-NH2 containing metabolites as well as the resulting diastereomers, and all the derivatized metabolites were resolved in a single anal. run. Data independent MS/MS acquisition (DIA) was applied to pos. identify DATAN-labeled metabolites based on reagent specific diagnostic fragment ions. We discriminated chiral from achiral metabolites based on the reversal of elution order of D and L isomers derivatized with the enantiomeric pair (±) of DATAN in an untargeted manner. Using the developed strategy, a library of 301 standards that consisted of 214 chiral and 87 achiral metabolites were separated and detected in a single anal. run. This approach was then applied to investigate the enantioselective metabolic profile of the bone marrow (BM) and peripheral blood (PB) plasma samples from patients with acute myeloid leukemia (AML) at diagnosis and following completion of the induction phase of chemotherapeutic treatment. The sensitivity and selectivity of the developed method enabled the detection of trace levels of the D-enantiomer of HAs and AAs in primary plasma patient samples. Several of these metabolites were significantly altered in response to chemotherapy. The developed LC-HR-MS method entails a valuable step forward in chiral metabolomics. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to untargeted chiral metabolomics liquid chromatog high resolution mass spectrometry, tandem, Placeholder for records without volume info and other aspects.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Nam, Jaehyuk; Jung, Yongseok; Jang, Woo-Dong published an article in 2017, the title of the article was Uracil-bearing poly(2-isopropyl-2-oxazoline): Hg(II)-selective control of its thermoresponsiveness.Related Products of 4433-40-3 And the article contains the following content:

A poly(2-isopropyl-2-oxazoline)-containing mercury ion (Hg2+)-responsive uracil moiety (U-PiPOx-U) was synthesized and it exhibited thermoresponsiveness in its aqueous solution The changes in the UV-visible absorption and thermoresponsiveness of U-PiPOx-U upon the addition of Hg2+ were studied. Selective sensing of Hg2+ was also studied. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Related Products of 4433-40-3

The Article related to uracil bearing polyisopropyloxazoline mercury selective thermoresponsiveness control, Inorganic Analytical Chemistry: Determinations and other aspects.Related Products of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Gackowski, Daniel; Gawronski, Maciej; Kerr, Cassandra; Radivoyevitch, Tomas; Zarakowska, Ewelina; Starczak, Marta; Abakir, Abdulkadir; Ruzov, Alexey; Maciejewski, Jaroslaw P.; Olinski, Ryszard published an article in 2020, the title of the article was Base 5-formylcytosine and 5-hydroxymethyluracil as surrogate markers of TET2 and SF3B1 mutations in myelodysplastic syndrome, respectively.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:

TET2 protein has been implicated in 5-hydroxymethyluracil generation to quantify the extent to which TET2 mutations cause deficiencies in TET2 dioxygenase activity. In the present study, the linkage between various TET2 mutations and 5-hydroxymethylcytosine, 5-formylcytosine, 5-carboxycytosine and 5-hydroxymethyluracil, and linkage between these modifications and myeloid neoplastic disease were characterized. We selected patients with a broad range of different types of TET2 alterations and detected in samples from them a broad spectrum of DNA modifications. Levels of DNA markers of TET2 activity obtained using automated two-dimensional ultra-performance liquid chromatog. tandem mass spectrometry with incorporation of stable isotope-labeled internal standards The results reavealed that the best predictor/marker of TET2 mutations in patients with myeloid malignancies was 5-formylcytosine. Receiver operating characteristic (ROC) curves for predicting TET2 mutated vs. wild-type subjects show that a 5-formylcytosine threshold of 0.204 per 106dN yields a sensitivity of 100% and a specificity of 83%. Disruption of SF3B1 in the K562 cell line resulted in G2/M cell cycle arrest. In conclusion, our results suggested that the anal. of 5-formylcytosine and 5-hydroxymethyluracil are warranted as surrogate markers of TET2 and SF3B1 mutations in myelodysplastic syndrome . The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to formylcytosine hydroxymethyluracil tet2 sf3b1 mutation myelodysplastic syndrome, Mammalian Pathological Biochemistry: Oncology and other aspects.Name: 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On March 31, 2019, Hlusicka, Jiri; Loster, Tomas; Lischkova, Lucie; Vaneckova, Manuela; Diblik, Pavel; Urban, Pavel; Navratil, Tomas; Kacer, Petr; Kacerova, Tereza; Zakharov, Sergey published an article.Product Details of 4433-40-3 The title of the article was Markers of nucleic acids and proteins oxidative damage in acute methanol poisoning. And the article contained the following:

Abstract: The aim of the study is to measure serum concentrations of markers of nucleic acids and proteins oxidative damage in humans to study the dynamics and clin. determinants of oxidative stress caused by acute methanol poisoning. Acute blood serum samples for this study were collected from 28 patients with methanol poisoning and the follow-up samples from 36 survivors of poisoning were collected 2 years after discharge. Serum concentrations of 8-hydroxy-2′-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-(hydroxymethyl)uracil (5-OHMU), ortho-tyrosine (o-Tyr), nitrotyrosine (NO-Tyr), and chlorotyrosine (Cl-Tyr) were measured by liquid chromatog.-electrospray ionization-tandem mass spectrometry. Acute concentrations of 8-OHdG and o-Tyr were significantly higher than the follow-up concentrations (94.4 ± 6.2 vs. 78.0 ± 10.0 pg cm-3; p = 0.009 and 163.0 ± 11.0 vs. 124.0 ± 17.0 pg cm-3; p < 0.001, correspondingly). Survivors of methanol poisoning had higher acute 8-OHdG and 8-OHG concentrations than those who died (97.3 ± 7.4 vs. 50.0 ± 23.0 pg cm-3; p < 0.001 and 97.9 ± 7.2 vs. 83.7 ± 6.7 pg cm-3; p = 0.047). Acute concentrations of 8-OHdG, 8-OHG, 5-OHMU, and o-Tyr were higher in the patients who survived without health sequelae than in those who survived with visual and CNS sequelae (all p < 0.05). Acute concentrations of markers of proteins and nucleic acids damage correlated with laboratory parameters of acidemia (anion gap) and serum ethanol concentration on admission (both p < 0.05). Acute elevation of the concentration of markers of nucleic acids and proteins oxidative damage in the patients with methanol poisoning suggest that mild-to-moderate oxidative stress may play an important role in the non-specific mechanisms of brain protection against direct neurotoxic effects of formic acid. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Product Details of 4433-40-3

The Article related to forensic biomarker nucleic acid protein oxidative stress methanol poisoning, Toxicology: Forensic Chemistry (Including Analysis) and other aspects.Product Details of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 31, 2022, Zhang, Hua; Zhao, Lin; Jiang, Jingjing; Zheng, Jie; Yang, Li; Li, Yanyan; Zhou, Jian; Liu, Tianshu; Xu, Jianmin; Lou, Wenhui; Yang, Weige; Tan, Lijie; Liu, Weiren; Yu, Yiyi; Ji, Meiling; Xu, Yaolin; Lu, Yan; Li, Xiaomu; Liu, Zhen; Tian, Rong; Hu, Cheng; Zhang, Shumang; Hu, Qinsheng; Deng, Yangdong; Ying, Hao; Zhong, Sheng; Zhang, Xingdong; Wang, Yunbing; Wang, Hua; Bai, Jingwei; Li, Xiaoying; Duan, Xiangfeng published an article.Category: pyrimidines The title of the article was Multiplexed nanomaterial-assisted laser desorption/ionization for pan-cancer diagnosis and classification. And the article contained the following:

As cancer is increasingly considered a metabolic disorder, it is postulated that serum metabolite profiling can be a viable approach for detecting the presence of cancer. By multiplexing mass spectrometry fingerprints from two independent nanostructured matrixes through machine learning for highly sensitive detection and high throughput anal., we report a laser desorption/ionization (LDI) mass spectrometry-based liquid biopsy for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) is applied in 1,183 individuals that include 233 healthy controls and 950 patients with liver, lung, pancreatic, colorectal, gastric, thyroid cancers from two independent cohorts. MNALCI demonstrates 93% sensitivity at 91% specificity for distinguishing cancers from healthy controls in the internal validation cohort, and 84% sensitivity at 84% specificity in the external validation cohort, with up to eight metabolite biomarkers identified. In addition, across those six different cancers, the overall accuracy for identifying the tumor tissue of origin is 92% in the internal validation cohort and 85% in the external validation cohort. The excellent accuracy and min. sample consumption make the high throughput assay a promising solution for non-invasive cancer diagnosis. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Category: pyrimidines

The Article related to liver lung pancreatic colorectal gastric thyroid cancer diagnosis biomarker, Biochemical Methods: Spectral and Related Methods and other aspects.Category: pyrimidines

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On September 30, 2018, Pelclova, Daniela; Navratil, Tomas; Vlckova, Stepanka; Fenclova, Zdenka; Pelcl, Tomas; Kacerova, Tereza; Kacer, Petr published an article.Synthetic Route of 4433-40-3 The title of the article was Exhaled breath condensate biomarkers reflect systemic changes in patients with chronic dioxin intoxication. And the article contained the following:

Abstract: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is highly toxic and affects the cardiovascular system, brain, and skin by AhR-dependent and other mechanisms, as well as causing metabolic impairments and cancer. The involvement of the respiratory system has not yet been studied. TCDD in the blood was measured and biomarkers of oxidative stress and inflammation were analyzed in 2016 in the exhaled breath condensate (EBC) of the last eight male survivors (mean age 72.4 years) from 80 workers intoxicated with TCDD during the production of herbicides from 1965 to 1968. The results were compared with their findings in 2010 to evaluate a trend. Malondialdehyde, 4-hydroxy-trans-nonenale, and 8-isoprostaglandin F2α (8-isoprostane), in addition to markers of the oxidation of nucleic acids and proteins 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine, 5-(hydroxymethyl)uracil, o-tyrosine, and 3-nitrotyrosine, as well as markers of inflammation leukotrienes and anti-inflammatory lipoxins, were analyzed in EBC by liquid chromatog.-electrospray ionization-tandem mass spectrometry. In addition, the patients underwent chest x-ray, spirometry and fractional exhaled nitric oxide (FeNO) examinations The control group included 7 men (66 years) with comparable lifestyle factors. The median plasma TCDD level lowered from 155 (28-553) ng/kg fat in 2010 to 112 (46-390) ng/kg fat in 2016, i.e., 50 years after exposure. The mean TCDD body deposit was 5.0 μg. Serum TCDD level in the pooled sample of the controls was 12 ng/kg fat. All markers of oxidative stress, LTB4 and LTC4, remained overexpressed in patients and anti-inflammatory lipoxins were under-expressed compared to controls (all). The mean FeNO and spirometry results were within the reference values. Borderline x-ray findings and combined lung function impairments were seen in the patients with the lower TCDD plasma levels. Differences in the expression of the biomol. markers in EBC as compared to controls were not associated with lung impairments and the respiratory parameters measured. Therefore, these EBC markers can be used to evaluate systemic oxidative stress and inflammation in tissues and the endovascular, atherosclerotic, neurotoxic, and metabolic effects of TCDD. Graphical abstract: [Figure not available: see fulltext.]. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Synthetic Route of 4433-40-3

The Article related to dioxin occupational poisoning breath condensate biomarker, Toxicology: Carcinogens, Mutagens, and Teratogens and other aspects.Synthetic Route of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On February 12, 2020, Chen, Feng; Xue, Jing; Zhang, Jin; Bai, Min; Yu, Xu; Fan, Chunhai; Zhao, Yongxi published an article.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Differentiated Visualization of Single-Cell 5-Hydroxymethylpyrimidines with Microfluidic Hydrogel Encoding. And the article contained the following:

5-Hydroxymethyluracil (5hmU) is found in the genomes of a diverse range of organisms as another kind of 5-hydroxymethylpyrimidine, with the exception of 5-hydroxymethylcytosine (5hmC). The biol. function of 5hmU has not been well explored due to lacking both specific 5hmU recognition and single-cell anal. methods. Here we report differentiated visualization of single-cell 5hmU and 5hmC with microfluidic hydrogel encoding (s.c.5hmU/5hmC-microgel). Single cells and their genomic DNA after cell lysis can be encapsulated in individual agarose microgels. The 5hmU sites are then specifically labeled with thiophosphate for the first time, followed by labeling 5hmC with azide glucose. These labeled bases are each encoded into resp. DNA barcode primers by chem. crosslinking. In situ amplification is triggered for single-mol. fluorescence visualization of single-cell 5hmU and 5hmC. On the basis of the s.c.5hmU/5hmC-microgel, we reveal cell type-specific mol. signatures of these two bases with remarkable single-cell heterogeneity. Utilizing machine learning algorithms to decode four-dimensional signatures of 5hmU/5hmC, we visualize the discrimination of nontumorigenic, carcinoma and highly invasive breast cell lines. This strategy provides a new route to analyze and decode single-cell DNA epigenetic modifications. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to fluorescent imaging cell microfluidic hydrogel, Biochemical Methods: Spectral and Related Methods and other aspects.Application In Synthesis of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia