Krell, Katja; Achim-Wagenknecht, Hans published an article in 2020, the title of the article was Fluorogenic and bioorthogonal modification of RNA using photoclick chemistry.Computed Properties of 4433-40-3 And the article contains the following content:
A bromoaryltetrazole-modified uridine was synthesized as new RNA building block for bioorthogonal, light-activated and postsynthetic modification with com. available fluorescent dyes. It allows “photoclick”-type modifications by irradiation with light (300 nm LED) at internal and terminal positions of presynthesized RNA with maleimide-conjugated fluorophores in good yields. The reaction was evidenced for three different dyes. During irradiation, the emission increases due to the formation of an intrinsically fluorescent pyrazoline moiety as photoclick product. The fluorogenecity of the photoclick reaction was significantly enhanced by energy transfer between the pyrazoline as the reaction product (poor emitter) and the photoclicked dye as the strong emitter. The RNA-dye conjugates show remarkable fluorescent properties, in particular an up to 9.4 fold increase of fluorescence, which are important for chem. biol. and fluorescent imaging of RNA in cells. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3
The Article related to phosphoramidite tetrazole rna fluorescent bioorthogonal photoclick chem, oligonucleotide, photochemistry, tetrazole, Placeholder for records without volume info and other aspects.Computed Properties of 4433-40-3
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia