On November 19, 2020, Zacharias, Martin published an article.Quality Control of 5-Methylpyrimidine-2,4(1H,3H)-dione The title of the article was Base-Pairing and Base-Stacking Contributions to Double-Stranded DNA Formation. And the article contained the following:
Double-stranded (ds)DNA formation and dissociation are of fundamental biol. importance. The neg. DNA charge influences the dsDNA stability. However, the base pairing and the stacking between neighboring bases are responsible for the sequence-dependent stability of dsDNA. The stability of a dsDNA mol. can be estimated from empirical nearest-neighbor models based on contributions assigned to base-pair steps along the DNA and addnl. parameters because of DNA termini. In efforts to sep. contributions, it has been concluded that base stacking dominates dsDNA stability, whereas base pairing contributes negligibly. Using a different model for dsDNA formation, we reanalyze dsDNA stability contributions and conclude that base stacking contributes already at the level of sep. ssDNAs but that pairing contributions drive the dsDNA formation. The theor. model also predicts that stability contributions of base-pair steps that contain only guanine/cytosine, mixed steps, and steps with only adenine/thymine follow the order 6:5:4, resp., as expected based on the formed hydrogen bonds. The model is fully consistent with the available stacking data and the nearest-neighbor dsDNA parameters. It allows assigning a narrowly distributed value for the effective free energy contribution per formed hydrogen bond during dsDNA formation of -0.72 kcal·mol-1 based entirely on the exptl. data. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).Quality Control of 5-Methylpyrimidine-2,4(1H,3H)-dione
The Article related to double stranded dna base pairing stacking model, General Biochemistry: Nucleic Acids and Their Constituents and other aspects.Quality Control of 5-Methylpyrimidine-2,4(1H,3H)-dione
Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia