Day: November 3, 2022

On September 26, 2013, Chan, Bryan; Estrada, Anthony; Shore, Daniel; Sweeney, Zachary published a patent.Recommanded Product: 4-(2-Bromopyrimidin-4-yl)morpholine The title of the patent was Preparation of pyrazolopyridines for treatment of Parkinsons disease. And the patent contained the following:

The title compounds I or II [R1 = (un)substituted alkyl, monocyclic heterocycloalkyl, bicyclic heterocycloalkyl, cycloalkyl; for I: 1-3 of X1-X4 = N, and the remainder are each CR2; for II: X4 = C or N, and 1-2 of X1-X3 = N and NR8, and the remainder = CR2, such that X1-X4 and N form a heteroaryl; R2 = H, alkyl, CN, halo, etc.; R8 = H, (un)substituted alkyl; with the proviso], useful for the prevention or treatment of a disorder caused by, associated with or accompanied by abnormal kinase activity, preferably abnormal LRRK2 activity, were prepared E.g., a multi-step synthesis of III, starting from 4-chloro-3-iodo-1H-pyrazolo[4,3-c]pyridine, was described. Exemplified compounds I and II were tested for their LRRK2 activity (data given). Pharmaceutical compositions comprising compound I or II, alone or in combination with other therapeutic agent, were disclosed. The experimental process involved the reaction of 4-(2-Bromopyrimidin-4-yl)morpholine(cas: 1209459-32-4).Recommanded Product: 4-(2-Bromopyrimidin-4-yl)morpholine

The Article related to pyrazolopyridine preparation lrrk2 inhibitor antiparkinsonian combination chemotherapy, Heterocyclic Compounds (More Than One Hetero Atom): Pyrazoles and other aspects.Recommanded Product: 4-(2-Bromopyrimidin-4-yl)morpholine

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On January 31, 2022, Khattab, Reham Abdel-Halim; Barghash, Safaa Mohamed; Mostafa, Osama Mohammad Sayed; Allam, Sahar Ali; Taha, Hoda Abdel-Halim; Ashour, Ameen Abd El-Baqi published an article.HPLC of Formula: 65-71-4 The title of the article was Seroprevalence and molecular characterization of Toxoplasma gondii infecting ruminants in the North-West of Egypt. And the article contained the following:

Toxoplasma gondii is a coccidian parasite known for its heavy toll on people and livestock. It can cause abortion and a variety of congenital diseases. The current study aimed to examine some seroprevalence and mol. attributes of T. gondii obtained from ruminants in the North-West of Egypt. Specimens were random selected from five different locations in Alexandria and Matrouh governorates. A total of 483 blood samples, collected from 96 mixed flocks, were screened for anti-T. gondii IgG antibodies using ELISA (ELISA). The seropos. results were then confirmed using polymerase chain reaction (PCR) primers for the B1 and P30 genes. Specific PCR products were selected for sequencing and alignment against the GenBank, where phylogeny has been examined using the maximum likelihood, neighbor-joining, and maximum parsimony in MEGA6. ELISA confirmed the presence of T. gondii in 188 of the investigated samples (38.92%), indicating a higher prevalence in camels (64.51%) and sheep (43.75%) as compared to goats (27.93%) and cattle (13.46%). PCR confirmed the presence of T. gondii-specific sequences in 159 seropos. specimens, with homol. between 98.3 and 100%. The genetic distances between the investigated variants ranged from 0.1 to 0.9, and 7 single nucleotide polymorphisms (SNPs), were identified in the examined T. gondii specimens. The camel T. gondii parasite, isolated from Matrouh, showed a 100% homol. with the most dangerous reference strains of T. gondii-RH in the GenBank. Our results showed that B1 and P30-specific PCR could detect T. gondii in blood samples more accurately than ELISA. In addition, the statistical anal. of our data indicated that species, age, sex, and animal location were all risk factors for toxoplasmosis. These findings are likely to boost disease control and help contain the spread of T. gondii infections. The experimental process involved the reaction of 5-Methylpyrimidine-2,4(1H,3H)-dione(cas: 65-71-4).HPLC of Formula: 65-71-4

The Article related to igg b1 p30 guanine thymine toxoplasma infection, b1gene, elisa, egypt, p30 gene, ruminants, toxoplasma gondii, Microbial, Algal, and Fungal Biochemistry: Classical Genetics and other aspects.HPLC of Formula: 65-71-4

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Rozalski, Rafal; Gackowski, Daniel; Siomek-Gorecka, Agnieszka; Banaszkiewicz, Zbigniew; Olinski, Ryszard published an article in 2016, the title of the article was Urinary Measurement of Epigenetic DNA Modifications: A Non-Invasive Assessment of the Whole-Body Epigenetic Status in Healthy Subjects and Colorectal Cancer Patients.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione And the article contains the following content:

Active mechanism of DNA demethylation can be responsible for the activation of previously silenced genes. Products of 5-methylcytosine oxidation are released into the bloodstream and eventually excreted with urine. Therefore, whole-body epigenetic status can be assessed non-invasively on the basis of the urinary excretion of a broad spectrum of epigenetic modifications: 5-hydroxymethylcytosine (5-hmCyt), 5-formylcytosine (5-fCyt), 5-carboxycytosine (5-caCyt), and 5-hydroxymethyluracil (5-hmUra). We have developed a specific and sensitive, isotope-dilution, automated, online, two-dimensional ultra-performance liquid chromatog. system with tandem mass spectrometry (2D UPLC-MS/MS) to measure 5-hmCyt, 5-fCyt, 5-caCyt, and their deoxynucleosides in the same urine sample. Human urine contains all of the modifications except from 5-formyl-2′-deoxycytidine (5-fdC) and 5-carboxy-2′-deoxycytidine (5-cadC). A highly significant difference in the urinary excretion of 5-(hydroxymethyl)-2′-deoxycytidine (5-hmdC) was found between healthy subjects and colorectal cancer patients (3.5 vs. 7.8 nmol mmol-1 creatinine, resp.), as well as strong correlations between the majority of analyzed compounds The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to liquid chromatog mass spectrometry epigenetic dna colorectal cancer urine, 2d uplc–ms/ms, dna damage, dna methylation, epigenetics, urine, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Reference of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On May 31, 2017, Sheng, Xiao-Qi; Wang, Yi-Chao published an article.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione The title of the article was Novel two-step derivation method for the synchronous analysis of inherited metabolic disorders using urine. And the article contained the following:

The aim of the present study was to conduct preliminary clin. screening and monitoring using a novel two-step derivatization process of urine in five categories of inherited metabolic disease (IMD). Urine samples (100 μl, containing 2.5 mmol/l creatinine) were taken from patients with IMDs. The collected urine was then treated using a two-step derivatization method (with oximation and silylation at room temperature), where urea and protein were removed. In the first step of the derivatization, α-ketoacids and α-aldehyde acids were prepared by oximation using novel oximation reagents. The second-step of the derivatization was that residues were silylated for anal. Urine samples were examined using gas chromatog./mass spectrometry (GC/MS) and a retention time-locking technique. The simultaneous anal. and identification of >400 metabolites in >130 types of IMD was possible from the GC/MS results, where the IMDs included phenylketonuria, ornithine trans-carbamylase deficiency, neonatal intrahepatic cholestasis caused by citrin deficiency, β-ureidopropionase deficiency and mitochondrial metabolic disorders. This method was demonstrated to have good repeatability. Considering a-ketoglutarate (α-KG) as an example, the relative standard deviations (RSDs) of the α-KG retention time and peak area were 0.8 and 3.9%, resp., the blank spiked recovery rate was between 89.6 and 99.8%, and the RSD was ≤7.5% (n=5). The method facilitates the anal. of thermally non-stable and semi-volatile metabolites in urine, and greatly expands the range of materials that can be synchronously screened by GC/MS. Furthermore, it provides a comprehensive, effective and reliable biochem. anal. platform for the pathol. research of IMDs. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

The Article related to inherited metabolic disease urine biomarker diagnosis gc ms, biomarker, inherited metabolic disorders, simultaneous, two-step derivatization, urine, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Quality Control of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On October 26, 2016, Shen, Weifeng; Han, Wei; Li, Yunong; Meng, Zhiqi; Cai, Leiming; Li, Liang published an article.Computed Properties of 4433-40-3 The title of the article was Development of chemical isotope labeling liquid chromatography mass spectrometry for silkworm hemolymph metabolomics. And the article contained the following:

Silkworm (Bombyx mori) is a very useful target insect for evaluation of endocrine disruptor chems. (EDCs) due to mature breeding techniques, complete endocrine system and broad basic knowledge on developmental biol. Comparative metabolomics of silkworms with and without EDC exposure offers another dimension of studying EDCs. The authors report a workflow on metabolomic profiling of silkworm hemolymph based on high-performance chem. isotope labeling (CIL) liquid chromatog. mass spectrometry (LC-MS) and demonstrate its application in studying the metabolic changes associated with the pesticide dichlorodiphenyltrichloroethane (DDT) exposure in silkworm. Hemolymph samples were taken from mature silkworms after growing on diet that contained DDT at four different concentrations (1, 0.1, 0.01, 0.001 ppm) as well as on diet without DDT as controls. They were subjected to differential 12C-/13C-dansyl labeling of the amine/phenol submetabolome, LC-UV quantification of the total amount of labeled metabolites for sample normalization, and LC-MS detection and relative quantification of individual metabolites in comparative samples. The total concentration of labeled metabolites did not show any significant change between four DDT-treatment groups and one control group. Multivariate statistical anal. of the metabolome data set showed that there was a distinct metabolomic separation between the five groups. Out of the 2044 detected peak pairs, 338 and 1471 metabolites have been putatively identified against the HMDB database and the EML library, resp. 65 metabolites were identified by the dansyl library searching based on the accurate mass and retention time. Among the 65 identified metabolites, 33 pos. metabolites had changes of >1.20-fold or <0.83-fold in one or more groups with p-value of smaller than 0.05. Several useful biomarkers including serine, methionine, tryptophan, asym. dimethylarginine, N-Methyl-D-aspartic and tyrosine were identified. The changes of these biomarkers were likely due to the disruption of the endocrine system of silkworm by DDT. This work illustrates that the method of CIL LC-MS is useful to generate quant. submetabolome profiles from a small volume of silkworm hemolymph with much higher coverage than conventional LC-MS methods, thereby facilitating the discovery of potential metabolite biomarkers related to EDC or other chem. exposure. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Computed Properties of 4433-40-3

The Article related to isotope labeling hplc mass spectrometry silkworm hemolymph metabolomics, isotope labeling, liquid chromatography, mass spectrometry, metabolomics, pesticide, silkworm, Biochemical Methods: Other (Not Covered At Other Subsections) and other aspects.Computed Properties of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 23, 2021, Arasappan, Ashok; Bell, Ian M.; Bungard, Christopher James; Burgey, Christopher S.; Cox, Jason M.; Kelly, Michael J., III; Layton, Mark E.; Liu, Hong; Liu, Jian; Perkins, James J.; Shah, Akshay A.; Vanheyst, Michael David; Wu, Zhe published a patent.Safety of 2,5-Dichloro-4-(trifluoromethyl)pyrimidine The title of the patent was 2-Oxoimidazolidine-4-carboxamides as NaV1.8 inhibitors and their preparation. And the patent contained the following:

Compounds of formula I, and the pharmaceutically acceptable salts thereof, are inhibitors of Nav1.8 channel activity and may be useful in the treatment, prevention, management, amelioration, control and suppression of diseases mediated by Nav1.8 channel activity. The compounds of the invention may be useful in the treatment, prevention or management of pain disorders, cough disorders, acute itch disorders, and chronic itch disorders. Compounds of formula I wherein one of A and B is (un)substituted aryl and (un)substituted heteroaryl, and the other one of A and B is (un)substituted aryl, (un)substituted heteroaryl, (un)substituted C1-6 alkyl-aryl, (un)substituted C3-8 cycloalkyl-aryl, etc.; R1, R2, R3 and R4 are independently H, (un)substituted C1-6 alkyl, (un)substituted C3-6 alkenyl, (un)substituted C3-6 alkynyl, (un)substituted C3-10 cycloalkyl, etc.; R5 is H and (un)substituted C1-6 alkyl; R6 is H, (un)substituted C1-6 alkyl, (un)substituted C3-6 cycloalkyl and (un)substituted C2-6 cycloheteroalkyl; R7 is H, (un)substituted C1-6 alkyl, (un)substituted C2-6 alkenyl and (un)substituted C2-6 alkynyl; and pharmaceutically acceptable salts thereof, are claimed. Example compound II was prepared by a multistep procedure (procedure given). The invention compounds were evaluated for their NaV1.8 inhibitory activity (data given). The experimental process involved the reaction of 2,5-Dichloro-4-(trifluoromethyl)pyrimidine(cas: 785777-98-2).Safety of 2,5-Dichloro-4-(trifluoromethyl)pyrimidine

The Article related to oxoimidazolidinecarboxamide preparation sodium channel inhibitor, Heterocyclic Compounds (More Than One Hetero Atom): Imidazoles and other aspects.Safety of 2,5-Dichloro-4-(trifluoromethyl)pyrimidine

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On January 1, 2018, Aybastier, Onder; Dawbaa, Sam; Demir, Cevdet published an article.Related Products of 4433-40-3 The title of the article was Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry. And the article contained the following:

Phenolic compounds have been studied elaborately for their efficacy to improve health and to protect against a wide variety of diseases. Herein this study, different anal. methods were implemented to evaluate the antioxidant properties of catechin and cyanidin using their standard substances and as they found in the grape seeds extracts Total phenol contents were 107.39 ± 8.94 mg GAE/g dw of grape seeds for grape seed extract (GSE) and 218.32 ± 10.66 mg GAE/g dw of grape seeds for acid-hydrolyzed grape seed extract (AcGSE). The extracts were analyzed by HPLC-DAD system and the results showed the presence of catechin, gallic acid, chlorogenic acid and ellagic acid in the processed methanolic extract and cyanidin, gallic acid and ellagic acid in the processed acidified methanolic extract The protective abilities of catechin and cyanidin were tested against the oxidation of DNA. The results showed that cyanidin has better protection of DNA against oxidation than catechin. GSE and AcGSE were revealed to inhibit the oxidatively induced DNA damage. GSE decreased about 57% of damage caused by the Fenton control sample. This study could show new aspects of the antioxidant profiles of cyanidin and catechin. The experimental process involved the reaction of 5-(Hydroxymethyl)pyrimidine-2,4(1H,3H)-dione(cas: 4433-40-3).Related Products of 4433-40-3

The Article related to dna oxidative damage grape seed extract antioxidant, antioxidant, catechin, cyanidin, dna oxidation, gc–ms/ms, grape seed, Food and Feed Chemistry: Fruits, Vegetables, Legumes, and Nuts and other aspects.Related Products of 4433-40-3

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On December 31, 2017, Kabal’nova, Natalia N.; Grabovskiy, Stanislav A.; Andriayshina, Nadezhda M.; Egorov, Vladislav I.; Valiullin, Lenar R.; Nabatov, Alexey A.; Raginov, Ivan S.; Murinov, Yurii I. published an article.Related Products of 626-48-2 The title of the article was The Impact of 5-Substituted Uracil Derivatives on Immortalized Embryo Lung Cells. And the article contained the following:

Background: Pyrimidine-based drugs stimulate tissue regeneration and immunity, two components that need to be improved in a number of respiratory diseases of different etiol. (e.g. influenza and asthma). In the present study we investigated relationships between the character of substitutions in the uracil structure and the impact of the resp. uracil derivatives on the immortalized lung cells. Methods: The level of cell proliferation, maximum tolerated dose and toxic effect of 5-substituted uracil derivatives (12 compounds) were studied on the immortalized lung epithelial cells and compared with the ones of 6-methyluracil. Results: 5-Carboxyuracil and 1,3-dimethyl-5-carboxyuracil had the lowest cytotoxicity among the studied compounds Their maximal tolerated dosage values were 5 times higher whereas the proliferation index was increased by 25% and 75%, resp., compared to 6-methyluracil, known for its pos. effects on cell regeneration. Conclusion: 5-Carboxyuracil and 1,3-dimethyl-5-carboxyuracil have the best perspectives for further studies on their biol. effects. The experimental process involved the reaction of 6-Methylpyrimidine-2,4(1H,3H)-dione(cas: 626-48-2).Related Products of 626-48-2

The Article related to uracil derivative immortalize embryo lung cell, Pharmacology: Effects Of Gastrointestinal and Respiratory Drugs and other aspects.Related Products of 626-48-2

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

Fitriana, Adita Silvia; Royani, Sri published an article in 2022, the title of the article was Molecular docking study of chalcone derivatives as potential inhibitors of sars-cov-2 main protease.Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione And the article contains the following content:

SARS-CoV-2 main protease is a potential target for the development of AntiCOVID-19. Several chalcones have inhibitory activity against 3CLpro SARSCoV and 3CLpro MERS-CoV. This study aims to predict the potential of chalcones in inhibiting 3CLproSARS-CoV-2, which plays a role in the viral replication process. In silico research carried the prediction through mol. docking toward proteins with PDB ID 6LU7 and 6Y2F. Compound K27 has a docking score more neg. than lopinavir. This result indicates that compound K27 is predicted to inhibit the SARS-CoV-2 replication. The experimental process involved the reaction of 6-Methylpyrimidine-2,4(1H,3H)-dione(cas: 626-48-2).Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione

The Article related to chalcone inhibitor protease mol docking sars cov2, Pharmacology: Effects Of Gastrointestinal and Respiratory Drugs and other aspects.Safety of 6-Methylpyrimidine-2,4(1H,3H)-dione

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia

On May 31, 2015, Dubovitskii, S. N.; Komissarova, N. G.; Shitikova, O. V.; Spirikhin, L. V.; Abdullin, M. F.; Yunusov, M. S. published an article.Computed Properties of 626-48-2 The title of the article was Synthesis of Betulin 28-(2-Bromoacetate) Conjugates with Uracil and its Methyl-Substituted Homologs. And the article contained the following:

A series of potentially biol. active betulin derivatives containing various C-28 2-uracilacetoxy substituents were synthesized [e.g., betulin bromoester I + uracil → II (88%)]. The experimental process involved the reaction of 6-Methylpyrimidine-2,4(1H,3H)-dione(cas: 626-48-2).Computed Properties of 626-48-2

The Article related to betulin uracil conjugate methyl homolog preparation, Terpenes and Terpenoids: Triterpenes (C30), Including Limonoids and other aspects.Computed Properties of 626-48-2

Referemce:
Pyrimidine | C4H4N2 – PubChem,
Pyrimidine – Wikipedia